Protein_Domain
SbpA

Part:BBa_K525401:Design

Designed by: Timo Wolf   Group: iGEM11_Bielefeld-Germany   (2011-09-10)

S-Layer SbpA from Lysinibacillus sphaericus


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 32
    Illegal BglII site found at 149
    Illegal XhoI site found at 1924
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 4
    Illegal AgeI site found at 3121
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 421
    Illegal BsaI.rc site found at 550


Design Notes

Truncated sbpA gene from Lysinbacillus spaericus CCM 2177 missing the first 30 amino acids

  • it was shown that these first 30 amino acid are not necessary for self-assembly


Source

  • Organism: Lysinbacillus spaericus CCM 2177
  • synthesized, codon-optimized for Escherichia coli


References

Badelt-Lichtblau H, Kainz B, Völlenkle C, Egelseer EM, Sleytr UB, Pum D, Ilk N (2009) Genetic engineering of the S-layer protein SbpA of Lysinibacillus sphaericus CCM 2177 for the generation of functionalized nanoarrays, [http://pubs.acs.org/doi/abs/10.1021/bc800445r Bioconjug Chem. 20(5):895-903].